Figure 5.
Antagonizing effect of naltrexone or β-funaltrexamine on morphine-induced HCV RNA expression in Huh.8 (A) and FCA-1 (B) cells. Huh.8 cells (2 × 104 cells/well) plated in a 96-well plate were incubated with naltrexone (10−8 mol/L) or β-funaltrexamine (10−8 mol/L) for 30 minutes before the addition of morphine (10−6 mol/L). Cell lysates then were subjected to real-time RT-PCR for HCV and GAPDH RNA quantification 72 hours after morphine treatment. The data are expressed as HCV RNA levels relative (Fold) to untreated control, which is defined as 1.0. The results shown are the mean ± SD of triplicate cultures, representative of five independent experiments (*, P < 0.01).