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. 2003 Oct;163(4):1395–1404. doi: 10.1016/S0002-9440(10)63497-9

Figure 4.

Figure 4.

Western immunoblotting. A: Extracts of human liver (lanes 1 and 7), skeletal muscle (lanes 2 and 8), thymus (lanes 3 and 9), lung (lanes 4 and 10), heart (lanes 5 and 11), and brain (lanes 6 and 12) were subjected to SDS-PAGE and transferred to membrane, and reacted with affinity-purified rabbit antibody to human skeletrophin peptide (lanes 1 to 6) or control antibody (lanes 7 to 11). A band with an approximate molecular weight of 70 kd was detected in human skeletal muscle, heart, and brain. In contrast, any significant band was observed with control antibody. B: Lysates from COS-7 cells, which were transfected with pCI-neo-skeletrophin (lane 1) or vector alone (lane 2), were subjected to SDS-PAGE, transferred to membrane, and reacted with anti-skeletrophin antibody. A major band with approximate molecular weight of 70 kd was detected in lysates of COS-7 cells transfected with pCI-neo-skeletrophin but not in those of COS-7 cells transfected with vector alone.