Figure 3.

A: Effect of Tam (1 × 10⁻⁷ M) on apoptosis in various mouse and human cells (MSG, HSG, MCF-7, Jurkat, HT-29, and Colo201). Staurosporin (1 μmol/L) was used as common apoptotic reagent. B: α-fodrin cleavage into 120 kd in MSG and HSG cells induced by Tam. Western blot analysis with mouse monoclonal Ab to α-fodrin was performed in MSG and HSG cells stimulated with Tam (1 × 10⁻⁷ M) for 24 or 48 hours. The data are representative of three individual experiments. C: Tam-induced apoptosis of MSG and HSG cells was significantly reduced by the pretreatment with estrogen. Apoptotic cells were detected by flow cytometer using propidium lodide staining and Annexin V-FITC. The data are the mean ± SD from three individual experiments.