Figure 4.

Validation of hrGal-1 effects in the chemotaxis assay. Purified PMNs were incubated for 5 minutes in the absence or presence of a specific anti-Gal-1 (α-Gal-1) or nonimmune IgG before the addition of 0.4 μg/ml of hrGal-1. A preparation of boiled hrGal-1 (0.4 μ/ml) was also tested. In both cases human recombinant IL-8 (30 ng/ml) was added to the lower chambers, and the number of PMNs migrated to the bottom well was assessed after 2 hours. Chemotaxis index was calculated as described in Figure 3 ▶ . Data are from cells prepared from three to four different donors. *, P < 0.05 versus respective vehicle group.