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. 2003 Oct;163(4):1255–1260. doi: 10.1016/S0002-9440(10)63485-2

Figure 3.

Figure 3.

CCNE1 and AKT2 fluorescence in situ hybridization (FISH). A: Interphase nuclei of the Su86.86 cell line showing a marker of 19p (TCF3/E2A, red) and extra copies of CCNE1 (green). B: Metaphase spread of Su86.86 showing TCF3 (red, 19p) and CCNE1 (green). Ectopic CCNE1 is shown with an arrow. C: Metaphase spread of normal cells showing single-copy signals for TCF3 (red, 19p), CCNE1 (green, 19q) and AKT2 (red, 19q). D: Metaphase spread showing amplification of AKT2 (red, q arm) and its centromeric neighbor, CCNE1 (green), in the Panc-1 cell line. TCF3 (red) is also shown as a marker of 19p. E: Southern blots of CCNE1 in the Su86.86, AsPC-1, and Panc-1 cell lines compared to levels in N57 (normal). The WI-12306 clone served as a loading control.