Figure 3.
Decreased induction of collagen α1(I) expression in FGF1(−/−)FGF2(−/−) livers after acute CCl4 treatment. Total liver RNA was isolated from three mice in each group at the indicated times after acute CCl4 treatment, pooled before assay by RNase protection, and relative intensity of the collagen α1(I) mRNA was quantitated and normalized as described in Materials and Methods. The signal in untreated WT livers was assigned a value of 1 and all other treatments expressed as a fold change over untreated wild type. P, labeled RNA probe. The displayed autoradiograph is representative of two independent reproductions.