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Normal activation of TGF-β1 expression in FGF1(−/−)FGF2(−/−) livers after acute CCl4 treatment. TGF-β1 mRNA was determined by RPA as described in Materials and Methods using 50 μg of pooled total RNA isolated from three livers. The intensity of TGF-β1 bands was quantitated as in Figure 3 ▶ and expressed relative to untreated WT assigned a value of 1. The displayed autoradiograph is representative of two independent reproductions.
