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. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2006 Dec;102(1-5):97–102. doi: 10.1016/j.jsbmb.2006.09.002

Figure 2. The Effect of MAPKinase Inhibitor, PD98059 on the Growth of MCF-7Ca and LTLT-Ca Cells.

Figure 2

MCF-7Ca and LTLT-Ca cells were transferred into steroid-free medium for 3 days before plating (1 × 104 cells per well) into 24-well plates. The next day, cells were washed with DPBS and treated with steroid-free medium containing androstenedione (25nmol/L) and the indicated concentrations of PD98059. The medium was changed every 3 days, and the cells were counted 9 days later using the MTT assay. Percentage of the PD98059 cells compared with the vehicle-treated cells (control). Expression of pMAPK is shown in the insert. (From Jelovac et al., Cancer Res., 2005).