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. Author manuscript; available in PMC: 2008 Jan 1.
Published in final edited form as: Anal Biochem. 2006 Oct 30;360(1):56–62. doi: 10.1016/j.ab.2006.10.01

Fig. 5.

Fig. 5

EC50 of taxol for tubulin-GDP in the presence of 15% DMSO (▲) and 4% DMSO (●). Tubulin-GDP samples (5μM) in PME buffer were incubated with different concentrations of taxol in 15% DMSO and 4% DMSO at 37 °C for 54 min. The percentage polymerization was measured in terms of the apparent absorption at 350 nm. The EC50 of taxol in 4% and 15% DMSO was 4.2 ±0.54 μM and 0.54 ± 0.16 μM respectively. The values are an average of three independent determinations.