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. Author manuscript; available in PMC: 2008 Feb 3.
Published in final edited form as: Biochem Biophys Res Commun. 2006 Dec 4;353(1):54–59. doi: 10.1016/j.bbrc.2006.11.110

Figure 4. α7 gene expression and promoter activity following osteogenic differentiation.

Figure 4

(A) BMP2-induced changes in integrin mRNA. Cells were cultured for 7 days as in Fig. 1 above with or without BMP2 and processed for RT-PCR using primers specific for GAPDH, α7, and α2. Compared to control cultures, BMP2 produced a decrease in α7 but induced α2 integrin under osteogenic conditions. (B) The α7 promoter activity was measured using the CAT receptor gene driven by wild-type α7 promoter (pCAT2.8). C2C12 myoblast cells were transiently transfected with the full length construct of mouse α7 integrin promoter and processed after treatment with BMP2. CAT promoter activity was measured and expressed as activity relative to that of empty vector pCAT. Transfection efficiency was normalized with co-transfection of the β-gal vector as an internal control. Data represents the mean of at least three separate experiments with error bar indicating S.D. * p < 0.01 vs. control; ** p < 0.005 as indicated by the bracket.