PI3KC2, PTEN, and SHIP do not accumulate on Hp phagosomes. (A) Forming IgG-Z phagosomes recruit PI3KC2 (arrowhead, left panel), but Hp phagosomes do not (right panel, Hp, red; PI3KC2, green). (B) Confocal sections of PMφ show PTEN or SHIP (green) and Hp (red). Arrowheads, Rare SHIP-positive Hp phagosomes. (C) Time course of PTEN and SHIP accumulation on IgG-Z phagosomes (arrowheads). (D) Differential recruitment of PI3KC2, PTEN, and SHIP to IgG-Z and Hp phagosomes. Data are the average ± SD of three independent experiments performed in triplicate. *, P ≤ 0.003, for Hp versus IgG-Z. p’somes, Phagosomes.