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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Anal Biochem. 2007 Jan 22;362(1):76–82. doi: 10.1016/j.ab.2006.12.015

Figure 2. Depletion of Tim44p or Tim23p disrupts protein import.

Figure 2

A. Western blots are shown for Tim17p, Tim23p, and Tim44p of inner membranes isolated from Tim23(Gal 10) and Tim44(Gal 10) yeast after 24 hours of growth with (+gal) or without (−gal) galactose. B, C. Autoradiographs: Radio-labeled preprotein Su9(1-69)DHFR was incubated for 5 minutes with mitochondria of Tim44(Gal 10) (B) and Tim23(Gal 10) (C) strains grown with or without galactose. Preprotein (p) and mature protein (m) were separated by SDS-PAGE and detected by autoradiography. Mature protein was quantified by densitometry. Plots show the amount of pSu9(1–69)DHFR imported as a function of time into mitochondria from Tim44(Gal 10) (D) and Tim23(Gal 10) (E) strains normalized to the amount imported after 15 minutes by mitochondria of that strain grown with galactose.