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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Anal Biochem. 2007 Jan 22;362(1):76–82. doi: 10.1016/j.ab.2006.12.015

Figure 3. Fluorescent signal peptides label mitochondria lacking Tim44p but not Tim23p.

Figure 3

Overlays of DIC and fluorescence images are shown of mitochondria from Tim23(Gal 10) (top) and Tim44(Gal 10) (bottom) strains after a 10 minute incubation under different conditions and 3 washes to remove peptides that were not imported. Mitochondria from yeast grown with (A) or without (B) galactose were incubated with Alexa Fluor-yCox-IV1-22 under energized conditions. Mitochondria from yeast grown with galactose were incubated with Alexa Fluor-yCox-IV1-22 plus CCCP (1 μM) (C) or with the control peptide Alexa Fluor-SynB2 under energized conditions (D). Scale bar in panel A corresponds to 10 microns for all panels. Squares in lower right corner of left panels show enlarged regions with 1 micron scale bar.