A, B, D. Typical current traces are shown of single TIM23 channels recorded from proteoliposomes containing inner membranes of mitochondria of indicated strains grown with (+gal) and without (−gal) galactose. O, S, C corresponds to the open (1000 pS), sub- (500 pS), and closed states, respectively. C. Inner membranes from Tim23(Gal 10) yeast were reconstituted with different protein concentrations. Plots show the frequency of detecting normal TIM23 channels as a function of total protein (left) and relative amount of Tim23p (right), which was determined by semi-quantitative western blots using densitometry. 12 independent patches/point are shown. D. Current traces are shown at +20 mV before (Control) and after sequential perfusion of the bath with 20 μM SynB2 and then 20 μM yCox-IV(1-13). E. Histograms of flicker rates (number of transition events/second) are shown in the absence (Control) and the presence of SynB2 or yCox-IV(1-13) for the TIM23 channels from Tim44(Gal10) yeast.