Table 1.
Comparison of the electrophysiological properties of TIM23 channels from control and mutant yeast strains.
| Wildtypea | +galb | Tim44(Gal 10) −gal | Tim23(Gal 10) −gal | |
|---|---|---|---|---|
| Peak conductance (pS) | 1160 ± 140
n=10 |
1002 ± 60
n=5 |
1008 ± 52
n=10 |
0
n=45 |
| Transition size (pS) | 490 ± 43
n=10 |
498 ± 31
n=5 |
500 ± 26
n=10 |
n.a. |
| Mean open time (ms) (+20mV) | 10.6± 4.3
n=13 |
12.0 ± 5.0
n = 19 |
13.1 ± 4.6
n = 5 |
n.a. |
| Voltage dependent | Yes | Yes | Yes | n.a. |
| Gating charge | −4.2 ± 0.7
n=20 |
−3.1 ± 0.4
n=19 |
−3.4 ± 0.5
n = 4 |
n.a. |
| V0 (mV)c | 50 ± 10
n=20 |
55 ± 10
n=19 |
59 ± 12
n = 5 |
n.a. |
| Permeability K+/Cl− | 5.0 ± 0.3
n=20 |
5.2 ± 1.4
n=11 |
5.9 ± 1.3
n = 5 |
n.a. |
| Peptide sensitivity | ↑Flicker | ↑Flicker | ↑Flicker | n.a. |
a
Data from Muro et al.(2003)[30].
b
Data compiled from both Gal10 strains grown in the presence of galactose
c
V0 voltage at which the channel spends half of the time open (Open probability, Po, is 0.5)
n is the number of independent patches, n.a. not applicable