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. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: J Autoimmun. 2006 Dec;27(4):218–231. doi: 10.1016/j.jaut.2006.12.002

Figure 2. Preventative coupled-cell tolerance to multiple myelin peptides prevents clinical EAE induced with a cocktail of encephalitogenic myelin peptides.

Figure 2

Female SJL mice were treated i.v. with 50×106 syngeneic splenocytes coupled to a myelin peptide cocktail consisting of PLP139–151, PLP178–191, MBP84–104, and MOG92–106 (Tolerized) or to a control peptide cocktail consisting of PLP56–70, OVA323–339, VP1233–250, and VP270–86 (Sham) seven days before priming. Nine to ten mice per group were immunized s.c. with a cocktail of 100 μg MBP84–104, 100 μg MOG92–106, 25 μg PLP139–151, and 50 μg PLP178–191/CFA on day 0. For DTH, mice were ear challenged with 10 μg of the indicated peptides on day 20, and swelling was measured 24 h later. *DTH response significantly less than sham-tolerized controls, p < 0.01. Data represents two separate experiments.