Figure 6. Therapeutic coupled-cell tolerance to multiple myelin peptides in established R-EAE induced by immunization with a cocktail of encephalitogenic myelin peptides ameliorates disease relapses.

Twenty-six female SJL mice were primed s.c. with a cocktail of myelin peptides consisting of 25 μg PLP_139–151, 50 μg PLP_178–191, 100 μg MBP_84–104, and 100 μg MOG_92–106 and CFA on day 0. On day 15 (indicated by arrow), at the peak of acute disease, mice were given 50×10⁶ syngeneic splenocytes coupled to a myelin peptide cocktail consisting of PLP_139–151, PLP_178–191, MBP_84–104, and MOG_92–106 (Tolerized) or to a control peptide cocktail consisting of PLP_56–70, OVA_323–339, VP1_233–250, and VP2_70–86 (Sham) i.v. and monitored for clinical disease (A) and peptide-specific DTH responses (B). For DTH, mice were ear challenged with 10 μg peptide on day 27, and swelling was measured 24 hours later. Data is representative of three separate experiments. *Responses significantly less than sham-tolerized controls, p < 0.05.