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. Author manuscript; available in PMC: 2007 May 15.
Published in final edited form as: Arch Biochem Biophys. 2007 Mar 7;461(2):186–193. doi: 10.1016/j.abb.2007.02.019

Fig. 1. PP2A activity and content in fetal and adult rat liver.

Fig. 1

Panel A: A liver cytosolic extract prepared from an adult male rat (5 μg homogenate protein per sample) was analyzed for PP2A activity in a time course experiment. The activity represents picomoles of free phosphate released from substrate at each time point. The unfilled square represents activity in the presence of 0.5 nM okadaic acid. Panel B: Fetal and adult liver extracts (n = 3 per group) were analyzed for PP2A activity (left). The reaction time was 15 min. *, p<0.02 by unpaired T-test. The same samples were analyzed for the intensity of the immunoreactive PP2A-C signal at 39 kDa as detected by the FL-C antibody (right). **, p<0.005 by unpaired T-test. Data in both graphs are shown as the mean plus 1 standard deviation. Panel C: Liver extracts prepared from fetal and adult liver were examined by Western immunoblotting using anti-PP2A-C antibodies directed towards full-length catalytic subunit (FL-C), the mid-portion of the protein (MD-C) and the carboxy-terminus (CT-C). The p85 subunit of PI3K was used as a control for quality and quantity of protein in each sample. The numbers to the right of the immunoblots indicate apparent molecular mass in kilodaltons.