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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2007 Mar;13(3):293–298. doi: 10.1016/j.bbmt.2006.12.440

Figure 1. Recipients containing CD8 TM specific for the single MiHA epitope resist engraftment.

Figure 1

A) Generation of anti-H60 CD8 TM. B6 mice sensitized 2X with B6 marrow-derived DC pulsed with H60 were analyzed for the presence of circulating CD8+ H60+ T cells 23 days following a booster injection. Gated CD8+H60TCR+ cells were analyzed for the expression of the TM markers CD44 and Ly 6C (A, histograms). B) Sensitized mice were irradiated at the indicated doses of TBI and 1 day later transplanted with syngeneic marrow. One day post-HCT, host spleen (left 3 panels) and marrow (right) were examined for the presence of CD8+ H60TCR+ cells. Numbers in dot plot reflect % of mononuclear cells; % CD8+H60TCR+ cells in spleen = 13.3 (3.0 Gy), 20 (6.0 Gy), 30.4 (9.0 Gy; BM = 25% at 9.0 Gy). C) One week post-HCT, peripheral donor chimerism (Ly 5.2, B6 gfp) was analyzed in DC-H60 sensitized B6 (CD45.1) mice ablatively conditioned (9.0 Gy) and transplanted with B6 H60 congenic 5 × 106 TCD-BM.