Early disappearance of calsequestrin mRNA in hind limb muscle after the up regulation of the TRDN transgene with Dox. A,B, E–H are in situ hybridization; C and D are the same sections as A and B immunostained with My32. A,C,E, and G are uninduced NF 55 transgenic tadpole limb sections; B,D,F, and H are limb sections of NF 55 transgenic tadpoles induced for 2 days with Dox. A,B) calsequestrin; E,F) actin; G,H) SERCA Ca(2+)-ATPase. Scale bar=200 μm