Table 2.
Primary candidate genes
| Accession number | Gene name | 2 wk | 6 wk | Frog | NF62 | In situ |
|---|---|---|---|---|---|---|
| BC043842 | phosphoglycerate mutase 2 | 0.13 | 0.28 | 56.91 | 0.03 | M+ |
| BC045082 | enolase 3 | 0.27 | 0.31 | 125.57 | 0.07 | M+ |
| BC043830 | phosphorylase kinase | 0.35 | 0.4 | 16.45 | 0.63 | M+ |
| AB003080 | troponin C, slow | 0.17 | 0.09 | 3.28 | NS | M+ |
| BX850052 | fast troponin I | 0.35 | NS | 8.48 | NS | M+ |
| M87307 | tropomyosin | 0.43 | NS | 14.20 | 0.36 | M+ |
| CD255184 | myosin heavy chain, E3 | 0.17 | 0.64 | 5.11 | 0.38 | M+ |
| BC046739 | actin, alpha 1 | 0.25 | 0.24 | 22.44 | 0.11 | M+ |
| BC042340 | LIM domain binding 3 | 0.48 | NS | 12.48 | 0.33 | M |
| BC041221 | LIM domain protein | 0.59 | NS | 14.71 | 0.10 | M+ |
| BC054224 | myozenin 1 | 0.34 | NS | 74.83 | 0.23 | M+ |
| BC046947 | calsequestrin 1 | 0.17 | 0.39 | 69.63 | 0.27 | M+ |
| BC044063 | ATPase, Ca++ | 0.29 | 0.57 | 7.07 | NS | M+ |
| BC042249 | creatine kinase M | 0.29 | NS | 8.61 | NS | M+ |
| CB945142 | creatine kinase B | 0.52 | NS | 24.70 | 0.33 | M |
| CF547537 | choline dehydrogenase | 0.35 | 0.35 | 9.18 | NS | |
| BX847508 | Cyt. c oxidase assembly | 0.39 | NS | 10.53 | 0.23 | M |
| BX851926 | ryanodine receptor 3 | 0.43 | NS | 4.85 | 0.37 | M |
| BC045081 | AchR alpha | 0.52 | 0.68 | 1.54 | 0.48 | M |
| U19612 | AchR epsilon | 0.53 | NS | 4.63 | 0.31 | M |
| BC043628 | AchR delta | 0.58 | NS | 2.36 | 0.21 | M |
| BJ029671 | sirtuin 1 | 0.57 | 0.62 | 25.62 | 0.23 | M |
| BC048222 | thrombospondin 3 | 0.57 | NS | 1.58 | 0.52 | |
| AY043260 | protein phosphatase 2a | 0.6 | NS | 1.62 | 0.26 | M |
| BC046852 | bridging integrator 1 | 0.49 | NS | 1.91 | 0.44 | M |
| BX851794 | Mg(2+) transporter | 0.51 | NS | 1.94 | 0.63 | |
| CB984281 | Mitoch. ADP/ATP translocase | 0.68 | NS | 5.90 | 0.24 | M |
| CD099943 | Unknown | 0.68 | 0.69 | 8.00 | NS | |
| BJ041930 | Unknown | 0.51 | NS | 1.85 | 0.41 | |
| BX854361 | ADP-ribosyltransferase 1 | 0.58 | NS | 11.60 | 0.51 | |
| AW764727 | FGF receptor substrate 3 | 0.47 | 0.63 | 9.97 | 0.23 | |
| BE026509 | Unknown | 0.38 | 0.31 | 2.48 | NS | |
| Selected secondary candidate genes: | ||||||
| BC046673 | aldolase A, fructose-bisphosphate | NS | NS | 16.21 | 0.19 | M |
| BC045015 | lactate dehydrogenase A | NS | NS | 10.66 | 0.20 | M |
| U39669 | pyruvate dehydrogenase | NS | NS | 5.35 | 0.38 | M |
| BC045100 | phosphofructokinase, muscle | NS | 0.61 | 23.60 | 0.16 | M |
| BC043781 | phosphoglycerate kinase 1 | NS | NS | 9.21 | 0.11 | M |
| BC046864 | triosephosphate isomerase 1 | NS | NS | 2.42 | 0.38 | M |
| BC054279 | EF1 alpha 2 | NS | NS | 338.77 | 4.95 | M |
M, known muscle expression; +, in situ confirmation of down regulation at the 6 wk time compared to a control at the same stage.
NS, not significant compared to 2 wk control