Table 1.
Dose-response data for meis3 MOs
| Morpholino1 | Embryos with ectopic insulin clusters2 | Deformed Embryos3 |
|---|---|---|
| tMO1 | ||
| 100uM | 22/38 (58%) | 0/38 (0%) |
| 200uM | 86/117 (74%) | 1/117 (1%) |
| 300uM | 22/31 (71%) | 3/28 (11%) |
| 400uM | 29/52 (56%) | 5/56 (9%) |
| tMO2 | ||
| 100uM | 22/63 (35%) | 0/63 (0%) |
| 150uM | 46/96 (48%) | 0/96 (0%) |
| MOCO | ||
| 100uM | 6/48 (12%) | 0/48 (0%) |
| 200uM | 18/240 (7%) | 0/240 (0%) |
| 300uM | 3/25 (12%) | 2/25 (8%) |
| 400uM | 2/29 (7%) | 3/29 (10%) |
1
Embryos were injected with the indicated MO concentration at the 1-cell stage, raised to 72hpf and scored for insulin expression by in situ hybridization.
2
Embryos with more than one patch of insulin expression were scored as having ectopic insulin clusters.
3
Deformed embryos showed convergence/gastrulation defects and were not scored for insulin expression.