Effect of desferioxamine supplementation on manganese-caused depletion of media DA and Dopac.Two ng each of DA and Dopac were incubated for 1 h in control medium, or in a medium containing MnCl2 (10, 100, 1000 μM) (Fig. 7a, c), or MnPO4 (1, 10, 100 μM) (Fig. 7b, d) with and without 1μM or 10μM desferioxamine (DES). Media levels of Dopac/DA were analyzed by HPLC-ECD. *, **, *** Presence and number of asterisks indicate Mn effect (P ≤ 0.05). “a”/”b” indicate DES effect within a Mn dose (P ≤ 0.05).