Table 2.
Methods for characterization of particular molecules
| Molecule | Function | Expression in non-activated EC | Common Test Method |
|---|---|---|---|
| Prostacyclin (PGI2) | anti-platelet aggregation | Low | Radio-immunoassay or enzyme immunoassay |
| Von Willebrand factor (vWF) | platelet adhesion | High storage but low secretion of active vWF | Immunostaining (expression and location)
ELISA on supernatant (amount secreted) |
| I-CAM, V-CAM, PECAM | blood cell adhesion | Low | Western blot (total expression)
Confocal, ELISA, flow cytometry (expression on cell surface) |
| Thrombomodulin (TM) | anti-fibrin generation | High | Immunostaining, ELISA (expression on cell surface)
Chromogenic assay (activity) |
| Endothelial cell protein C receptor (EPCR) | anti-fibrin generation | High | Western blot (total expression)
Immunostaining, ELISA (expression on cell surface) |
| Tissue Factor (TF) | fibrin generation | Low | Immunostaining, ELISA (expression on cell surface)
Chromogenic assay (activity) |
| Tissue factor pathway inhibitor (TFPI) | anti-fibrin generation | High | Chromogenic assay (activity) |
| Tissue plasminogen activator (tPA) | fibrinolysis | Low | Western blot (total expression)
ELISA (amount secreted) Chromogenic assay (activity) |
| Plasminogen activator inhibitor-1 (PAI-1) | anti-fibrinolysis | High | Western blot (total expression)
ELISA (amount secreted) Chromogenic assay (activity) |