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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Biomaterials. 2007 Feb 9;28(16):2547–2571. doi: 10.1016/j.biomaterials.2007.01.039

Table 4.

Molecular based studies suggesting substrate affects EC thrombogenicity.

Molecule tested Cell type Substrate material results Ref
TM, vWF PECAM VE-cadherin Human umbilical vein TCPS
Decellularised fibroblast matrix		 On decellularised matrix more PECAM, VE-cadherin and lower vWF. Also TM higher and constant over time compared to TCPS on which TM decreased over time. 156
TM, vWF Human umbilical vein albumin and sulphated chitosan coated polyester, TCPS On polyester TM lower and vWF secretion higher 157
PGI2 Bovine aortic Fn, Laminin (of varying concentration) and gelatin coated TCPS PGI2 increased post confluence on Fn and laminin coatings but not gelatin. Laminin conc. controlled pre and post confluence secretion levels 173
PGI2 Bovine aortic
Porcine aortic		 TCPS coated with different concentration of poly-HEMA to control “adhesivity” On higher “adhesivity” surface more distinct F-actin structure and higher PGI2 secretion 166
PGI2, vWF Human umbilical vein Fn-TCPS, crosslinked and non-crosslinked collagen Small but significant difference in PGI2 and vWF secretion on TCPS versus crosslinked collagens 140
tPA, PGI2 Human saphenous vein ePTFE with or without coating of Fn, collagen-1 or mixture of both Higher levels of PGI2 and tPA on Fn-ePTFE and ePTFE versus samples with collagen or collagen-Fn 167
PGI2, PAI-1, tPA Human saphenous vein Plastic, gelatin, collagen-1 gel, Fn, fibrin glue, de-endothelized porcine aorta, ePTFE precoated with collagen-I No difference in basal levels of PGI2. tPA secretion significantly higher on collagen-1 gels but not on the collagen-1 precoated ePTFE. PAI higher on the collagen 1, gelatin, fibrin glue and porcine aorta. 158
PGI2, PAI-1, tPA Human umbilical vein PET, polypropylene On PET significant less PGI2 and PAI-1 compared to polypropylene but tPA no different. 168
PGI2, PAI-1, tPA Human umbilical vein Ammonia plasma modified PTFE coated with Fn, collagen-1 or gelatin Different levels of PGI2, PAI-1 and tPA between coatings and unmodified versus modified PTFE. 148
tPA and PAI-1 Human umbilical vein TCPS, PTFE and polyurethanes all coated with Fn On Fn coated PTFE and polyurethanes higher secretion of PAI and tPA than on fn –TCPS, More PAI-1 deposited in underlying matrix on PTFE 149
PGI2, PAI-1, tPA Human umbilical vein Woven Dacron, double velour Dacron, Dacron-urethane and carbon coated Dacron urethane Variations seen in PGI2 among surfaces. tPA secretion higher on Dacron-urethane and PAI-1 levels similar on all surfaces 159
tPA, PAI-1, vWF Human umbilical vein TCPS and PVC coated with gelatin, collagen and Fn On PVC substrates, tPA increased, vWF secretion decreased and PAI-1increased (only PVC coated with collagen or gelatin). 169
ICAM Human umbilical vein
Human saphenous vein		 Fn-ePTFE, PTFE, Fn-TCPS, TCPS ICAM expression increased on ePTFE surface 160,161, 162
ELAM-1 Human umbilical vein PET with or without layer or pyrolytic carbon ELAM-1 lower on PET with carbon layer 215,216
P-selectin Human umbilical vein Knitted PET, TCPS P-selectin increased on knitted PET 189
ICAM, VCAM Human umbilical vein PS or TCPS both coated with Fn No difference in ICAM but VCAM lower on PS. 174

Fn = fibronectin, PS = polystyrene, TCPS = tissue culture PS, PET = poly(ethylene terephthalate)