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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Clin Immunol. 2006 Dec 19;123(1):18–29. doi: 10.1016/j.clim.2006.10.014

Figure 5. Effector functions are differentially regulated during Treg induction.

Figure 5

Allostimulated CD4+CD25− T-cell cultures were flow sorted for CD4+, CFSEhigh, CD25+ fraction (Day 2), CD4+, CFSElow, CD25+, dividing fraction (Days 4, 6 and 9) to obtain highly pure induced Treg populations at different stages of their formation. FOXP3, IFN-γ, TNF-α and IL-10 messages were quantified by real time PCR and normalized to β-actin. Presence of specific product was confirmed by dissociation curve and gel analysis.