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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Clin Immunol. 2006 Dec 19;123(1):18–29. doi: 10.1016/j.clim.2006.10.014

Figure 6. CD8+CD25− T-cells show transient FOXP3 upregulation and suppressor activity following activation.

Figure 6

a, Gated CD8+ T-cells from an allostimulated CD3+CD25− T-cell culture are shown. Transient upregulation of FOXP3 and CD25 on CFSElow (dividing) cells is depicted. b, CD25+ T-cells were sorted from 5-day cultures of anti-CD3-stimulated CD8+CD25− T-cells and tested for their ability to suppress autologous CD4+CD25− T-cells in 3H-thymidine based suppression assays. Proliferation data was normalized to responder proliferation (100%), which ranged between 50,000 to 80,000 CPM in various experiments. c, Suppressive ability of flow-sorted CFSElow (dividing) cells from day 4 and day 10 of anti-CD3-activated CD8+CD25− T-cell cultures.