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. Author manuscript; available in PMC: 2007 May 14.
Published in final edited form as: J Biosci. 2003 Feb;28(1):83–89. doi: 10.1007/BF02970136

Figure 2.

Figure 2

Metalloporphyrins also inhibit 6-OHDA cell injury. B65 cells were exposed to 500 μM 6-OHDA for 20 h. Thirty minutes prior to the addition of 6-OHDA or vehicle, media was replaced with fresh media alone (Media), HEPES (the diluent for the antioxidants) or media containing SOD (150 U/ml), catalase (30 U/ml, Cat), MnTBAP (100 μM, TBAP), or MnTE-2-PyP (T2E, 50 μM). Cell injury was determined by determining the percent of LDH released into the media. The baseline percent release from ascorbate (the vehicle for 6-OHDA) treated cells was 11.7 ± 0.4%. *P < 0.05 by two-tailed Student’s t-test as compared to cells exposed to 6-OHDA in the absence of catalytic antioxidants. Specific activities of Mn-TBAP and MnTE-2-PyP were 60,000 and 3,000,000 U/mmol respectively, and of catalase, 260,000 U/ml and of SOD, 4,600 U/ml. A representative plot is illustrated.