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. Author manuscript; available in PMC: 2007 May 14.
Published in final edited form as: J Neurochem. 2001 May;77(4):1058–1066. doi: 10.1046/j.1471-4159.2001.00304.x

Fig. 5.

Fig. 5

Effect of the MEK inhibitor PD98059 upon 6-OHDA mediated ERK phosphorylation. B65 cells treated for 20 h with 500 mM 6-hydroxydopamine (6-OHDA) in the presence (+) or absence (−) of 50 μM PD98059 were lysed and equal amounts of protein (50 μg) were subjected to immunoblot analysis using antibody against the activated form of ERK (top, P-ERK). The blots were then stripped and reprobed with antibody against total ERK (bottom, T-ERK).