Table II. ER–plasma membrane junction (peripheral coupling) statistics.

Cell type	Average length of junction (mean ± SD)	No. of junctions/cell profile
(A) Sorted GFP-expressing T3-HEK293 cells	218 ± 94 nm (n = 165 junctions)	0.5/cell (n = 112 cells)
(B) Sorted GFP-junctate–expressing T3-HEK293 cells	339 ± 51 nm (n = 126 junctions)*	1/cell (n = 100 cells)**
(C) Sorted GFP-pShag-RNAi-junctate T3-HEK293 cells	139 ± 51 nm (n = 15 junctions)***	0.1/cell (n = 108 cells)****

t test: A versus B,

^*P < 0.0001; A versus B,

^**P < 0.007; A versus C,

^***P < 0.002; A versus C,

^****P < 0.009.