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. 1999 Jul;155(1):315–324. doi: 10.1016/S0002-9440(10)65125-5

Figure 1.

Figure 1.

A: diagram showing the structure of the URR-EJras transgene. The URR-EJras transgene contains a 0.76-kb regulatory fragment (URR) from CRPV and a 2.1-kb coding sequence of EJras. The fragment of CRPV URR (diagonal box) was ligated to the coding sequence of EJras, which contains all of the exons (black box), introns (open box), and a 3′ flanking sequence carrying the polyadenylation signal as indicated. B: Southern blot analysis of EJras in the transgenic founders. Each lane contains 10 μg DNA, which was isolated from the biopsies of the rabbit ear skin and digested by BamHI and EcoRI. DNAs were isolated from transgenic founders TR-ras1 (lane 1) and TR-ras2 (lane 2) and four nontransgenic rabbits (lanes 3 to 6). DNA in lane 7 was the SalI- and EcoRI-released URR-EJras DNA reconstructed with normal rabbit skin DNA as a control. The two fragments produced by BamHI (EcoRI does not cut unintegrated URR-EJras) digestion in lane 7 indicate the appropriate separation of URR (0.76 kb) and the EJras coding sequence (2.1 kb). The fragment patterns shown in lanes 1 and 2 indicate that the TR-ras 1 rabbit may contain a single copy of integrated URR-EJras, and the TR-ras 2 rabbit contains multiple copies of the integrated URR-EJras, with head-to-tail tandem repeats.