Figure 4.
Ethidium bromide-stained gels of the RT-PCR experiments for amplification of β1C (A) and β1A (B) mRNA of six breast carcinomas. Lack of amplifiable β1C mRNA in four breast carcinomas (lanes 1–4) correlated with the negative immunohistochemical and immunoblotting results. The two tumors showing amplified β1C mRNA (lanes 5 and 6) were also immunoreactive in immunocytochemistry and immunoblotting experiments. Lane 7: Normal breast tissue. Lane 8: Normal cell line (MCF10). Lane 9: Positive control (normal prostate). Lane 10: Negative control (no complementary DNA).