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. 2000 Jan;156(1):77–90. doi: 10.1016/S0002-9440(10)64708-6

Figure 6.

Figure 6.

Distribution of phosphorylated CK8 or CK18 and total CK8/18 in intoxicated livers of mice fed a DDC-containing diet for 2.5 months (2.5 m DDC). Immunofluorescence double-labeling was performed on frozen tissue sections using antibodies to phospho-CK8 (5B3, LJ4; green in a–d), phospho-CK18 (8250; red in e, f), and to total CK8/18 (red in a, c; green in e). Double- (D-IF) and corresponding single- (S-IF) label confocal micrographs are shown. 5B3, LJ4, 8250: In many hepatocytes cytokeratin filaments and MBs (arrowheads) are brightly stained with antibodies to phospho-CK8/18. Note that in some hepatocytes only MBs are immunoreactive, whereas the cytokeratin IF network remains unstained (asterisk in c and d). Red aggregates in e and f, also present in negative controls, represent autofluorescent porphyrin deposits (see Figure 4 ▶ ). Bar = 20 μm.