Skip to main content
. 2000 Jan;156(1):77–90. doi: 10.1016/S0002-9440(10)64708-6

Figure 9.

Figure 9.

Phosphorylation of CK8 in DDC-intoxicated mouse livers. Cytoskeletal fractions were prepared from livers of control and DDC-intoxicated mice. Equal amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electroblotted onto nitrocellulose membranes, and then stained with antibodies to phospho-CK8 (5B3, LJ4) and to the total CK8 pool (Ks 8.7). The Western blot is shown in A and the densitometric analyses of the band intensities using a Docu Gel V video densitometer (MWG Biotech, Ebersberg, Germany) and Rflp-scan software (MWG Biotech) are shown in B. The bars indicate the relative amount of phosphorylated CK8 as detected by 5B3 and LJ4 normalized to total CK8 as revealed by Ks.8.7. Abbreviations: n.d., not detectable; CO, control diet; 1 w DDC, DDC-containing diet for 1 week; 2.5 m DDC, DDC-containing diet for 2.5 months; Recovery, 1 month control diet after 2.5 months of DDC-feeding; Reintox., DDC-feeding for 3 days after 1 month recovery.