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Effect of the hydroxyl radical scavengers mannitol (10 mmol/L) and 2-mercaptoethanol (300 μmol/L) on the anti-angiogenic effects exerted by thalidomide. A: Control sample, treated with the solvent DMF: B: Thalidomide (100 μg/ml) treated sample. C: Sample treated with mannitol (10 mmol/L) and thalidomide (100 μg/ml). D: Sample treated with 2-mercaptoethanol (300 μmol/L) and thalidomide (100 μg/ml). The capillary areas of representative embryoid bodies are shown. Scale bar, 200 μm.
