Table 2.
In Vitro and in Vivo Sensitivity of ISET
| No. of cells detected by ISET | |||
|---|---|---|---|
| Hep G2 | LNC aP | MCF-7 | |
| a. In vitro tests | |||
| No. of micropipetted cells | |||
| 3 | 3 (−) | 1 (−) | 3 |
| 3 (−) | 3 (−) | 3 | |
| 3 (−) | 3 (−) | 0 | |
| 1 (−) | 3 (−) | 3 | |
| 3 (−) | 2 (−) | 3 | |
| 1 | 1 (−) | 1 (−) | 1 |
| 1 (−) | 1 (−) | 1 | |
| 0 (−) | 1 (−) | 0 | |
| 1 (−) | 1 (−) | 1 | |
| 1 (−) | 1 (−) | 0 | |
| b. In vivo tests in patients with liver cancer |
| No. of tumor cells (*) and tumor microemboli (°) detected by ISET | ||
|---|---|---|
| Patient | Before surgery | During tumor resection |
| 1 | 4° (−) | 3*+ 5° (−) |
| 2 | 0 (−) | 1* (−) |
| 3 | 0 (−) | 1*+ 12° (−) |
| 4 | 1° (−) | 0 (−) |
| 5 | 0 (−) | 2* (+) |
| 6 | 1° (−) | 2° (+) |
| 7 | 0 (−) | 2* (+) |
RT-PCR results are in parentheses. ISET tests were performed in 1 ml peripheral blood samples. RT-PCR in vitro and in vivo tests were performed in 1 and 3 ml of blood, respectively.