Fig. 1.

DLC1 and tensin form a complex in mammalian cells. (A) DLC1 was cotransfected with GFP or GFP-tensin into 293 cells, and specifically detected DLC1 was coimmunoprecipitated in the anti-GFP immunopellet (Left). Reciprocally, GFP-tensin transfected into H1299 cells was specifically detected by coimmunoprecipitation of endogenous DLC1 with a DLC1 antibody (Right). (B) Extracts from mel 1011 cells, which contain endogenous DLC1 and tensin, were immunoprecipitated with control IgG or a tensin antibody. Coprecipitated DLC1 was detected by a DLC1 antibody (Upper). The blot was reprobed with the tensin antibody (Lower).