Fig. 3.

DLC1 interferes with proteins that bind the tensin SH2 domain in Src-transformed cells. v-Src-transformed 3T3 cells were cotransfected with the wild-type GST-SH2 domain with or without wild-type or mutant DLC1 as indicated. Transformed cells were also transfected with mutant GST-SH2 domains as indicated. Untransformed 3T3 cells were transfected with wild-type GST-SH2 domain. After Gluta pull-down, the proteins bound to the SH2 domain were detected by anti-pY blot (PY; Top). The expression of transfected GST fusion proteins and DLC1 is shown in Middle and Bottom, respectively.