Figure 1.

Differential hybridization of candidate maternally expressed genes. The control β-actin gene that is biallelically expressed and the Peg1/Mest gene that is paternally expressed were removed after three cycles of subtraction. The positive control, H19, which is expressed maternally is shown as Meg2 (H19). Thirty nanograms of each DNA from clones representing Meg1 and Meg3–7 were spotted onto filter membranes and hybridized with F, A, and S probes, respectively. Because of the very high level of expression of the Meg2 (H19) gene, only 1.5 ng of DNA was spotted.