Figure 6.

Cohesive analysis of DNA copy number changes and differential gene expression in the mouse megabladder model. The region of 25.9 to 28.0 Mb on mouse chromosome 16 contains 10 genes; amplification of five of these genes in homozygous mutants of the megabladder mouse was observed by graCNV (A) and confirmed by quantitative PCR (B). Differential gene expression between homozygous mutants and wild type shows overexpression of four of the amplified genes in whole embryos (C) and overexpression of three genes in the target organ, the embryonic bladder (D). (E) Interphase FISH analysis of a homozygous megabladder mutant shows two copies of chromosome 11 (green) and four copies of the duplicated region of chromosome 16 at 27.0 Mb (red).