Figure 3.

Prostaglandin F_2α and E₂ production by BEE cells over-expressing PLA2G4C. Culture medium from BEE cells, transfected with PLA2G4C cDNA, was harvested 6 h after exposure to treatments and concentrations of PGs were analyzed by ELISA. Treatments included Control, C; IFNT, I; Oxytocin, O, Oxytocin and IFNT, O&I. Data are expressed as means and SEM. Treatments were performed in triplicate and repeated (n = 6). Columns with different superscripts are significantly different (p < 0.05). Western blot at top of figure compares PLA2G4C expression in transfected cells (lane 1) and non-transfected cells (lane 2).