Figure 5.
BEE cells exhibit both Ca++-dependent and Ca++-independent PLA2 activities. PLA2 activity assays were performed on cellular lysates following treatment of intact cells, as described in the Methods. Ca++-dependent PLA2 activity assays were performed with AA-PC and 14C-AA-PC in the presence of 5 mM CaCl2and Ca++-independent assays were performed using the same substrates but in the absence of CaCL2 and the presence of 5 mM EGTA. Treatments included the PLA2G6 inhibitor BEL, the PLA2G4 and PLA2G6 inhibitor MAFP and the PLA2G4A inhibitor PYR-1. Data from columns with different superscripts are significantly different (p < 0.05).