Figure 1. Efficiency of LysCre- and MXCre-mediated loxP recombination in peritoneal macrophages, BM-derived macrophages, and hepatic Kupffer cells.

(A) PCR analysis of genomic DNA isolated from the indicated tissues, BM, BM-Mφ, and TG-Mφ of conditionally targeted LysMCre⁺PPARγ^f/f (MAC-KO) mice. (B) Western blot analysis of protein isolated from peritoneal macrophages harvested from MAC-WT and MAC-KO mice. (C and D) PCR analysis of genomic DNA from purified Kupffer cells harvested from MAC-WT and MAC-KO mice. (E) PCR analysis of genomic DNA isolated from hematopoietic tissues (liver, spleen, BM, BM-Mφ, and TG-Mφ) from MXCre^–PPARγ^f/f control and conditionally targeted MXCre⁺PPARγ^f/f donor mice after poly-I:C induction. (F) PCR analysis of genomic DNA isolated from circulating blood leukocytes from BMT MAC-WT and BMT MAC-KO mice 4–6 weeks after BMT.