Figure 3. Macrophage-specific PPARγ gene deletion causes impaired insulin signaling and lipid accumulation in skeletal muscle and liver.

Skeletal muscle (quadriceps) and liver samples were harvested from BMT MAC-WT (n = 6; white bars) and BMT MAC-KO (n = 6; black bars) mice following the glucose clamp, and homogenates were immunoprecipitated and immunoblotted for the quantification of IRS-1 tyrosine phosphorylation (A) as well as for Akt1/2 (Ser473), (C) IKK-α/β (Ser180/181), and (D) JNK (Thr183/Tyr185) phosphorylation relative to protein content (B). Muscle and liver samples were also dehydrated and analyzed for the accumulation of lipid intermediates including diacylglycerol (DAG), long-chain fatty acyl-CoA (LCFACoA) (E), and triacylglycerol (F). All values are expressed as a mean ± SEM. Mean differences were detected using 1-way ANOVA; *P < 0.05.