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. 2007 May 14;404(Pt 2):345–351. doi: 10.1042/BJ20061609

Figure 1. Modular kinetic analysis: validation of the method.

Figure 1

The Figures shows a modular kinetic analysis of the kinetic responses of the three modules of oxidative phosphorylation [substrate oxidation, circles; proton leak, squares; Δψ-consumers (sum of phosphorylating system and proton leak), triangles] to membrane potential, Δψ, in mitochondria isolated from cybrids, using 4 mM succinate as substrate. (A) Kinetic responses in mitochondria isolated from A, D, L1 and L2 cybrids in the absence (closed symbols) or presence (25 nM; open symbols) of the Complex III inhibitor myxothiazol. Results are means±S.E.M. for four independent mitochondrial preparations. The kinetics of substrate oxidation were significantly different at membrane potentials above 98 mV. (B) Kinetic responses in mitochondria isolated from cybrids harbouring a pathogenic mutation at np 3243 in the tRNA-Leu(UUR) gene (MTTL1). Closed symbols, clone Z (high proportion of wild-type); open symbols, clone X (high proportion of mutant). Results are means±S.E.M. for three independent mitochondrial preparations. The kinetics of substrate oxidation were significantly different at all membrane potentials.