(A) Induction of a LexA-regulated gene(s) other than or in addition to recA is required for efficient Lac+ adaptive mutation. ○, recAo281; □, rec+; ◊, lexA3(Ind−); ▵, lexA3(Ind−) recAo281. (B) umuDC is not required for adaptive mutation. □, Δ(umuDC)595∷cat; ◊, umu+. In this and all figures, all strains shown together were tested in parallel, and the means ± SEM (error bars) of several independent cultures tested in parallel are displayed.