Table 1. Phosphorylation sites identified within A. thaliana wild-type and abi1-1 mutant cruciferins by electrospray ionization MS/MS.
pS, phosphoserine; pT, phosphothreonine; pY, phosphotyrosine.
| Peptide and phosphorylation site(s) | Locus* | Subunit | Source |
|---|---|---|---|
| EAPFPNACHFpSQINSLAPAQATK | At1g03890.1 | α | abi1-1 |
| pSEAGQIEYWDHNHPQLR | At4g28520.1 | α | abi1-1 |
| VFHLAGNNQQGGFGGpSQQQQEQK | At4g28520.1 | α | abi1-1 |
| VVPGCAEpTFMDpSQPMQGQQQGQPWQGR† | At4g28520.1 | α | abi1-1 |
| IpSYVVQGTGISGR | At4g28520.1 | α | WT/abi1-1 |
| VIPGCAEpTFMESPVFGEGQGQGQSQGFR | At1g03880.1 | α | WT/abi1-1 |
| VIPGCAEpTFQDSSEFQPR | At5g44120.3 | α | WT/abi1-1 |
| pYVIEQGGLYLPTFFTSPK | At4g28520.1 | α | WT/abi1-1 |
| FpSTIETTLTHSSPMSYGRPR | At1g03880.1 | β | WT/abi1-1 |
| VFDQEISpSGQLLVVPQGFSVMK | At1g03880.1 | β | WT/abi1-1 |
| ADVYKPQLGYIpSTLNSYDLPILR | At5g44120.3 | β | abi1-1 |
| ADVYKPQLGpYISTLNSYDLPILR | At5g44120.3 | β | abi1-1 |
| ALPLEVIpSNGFQISPEEAR | At4g28520.1 | β | abi1-1 |
| CTENLDDPSDADVpYKPSLGpYISTLNSYNLPILR‡ | At1g03880.1 | β | abi1-1 |
| FNpTLETTLTR | At4g28520.1 | β | abi1-1 |
| GLPLEVIpTNGFQISPEEAR | At5g44120.3 | β | abi1-1 |
| GLPLEVIpTNGYQISPEEAK | At1g03880.1 | β | abi1-1 |
| pTNENAMISTLAGR | At4g28520.1 | β | abi1-1 |
| pTNENAQVNTLAGR | At1g03880.1 | β | abi1-1 |
| VTpSVNSYTLPILEYVR | At4g28520.1 | β | abi1-1 |
* Denotes locus and gene model corresponding to cruciferin from which the tryptic peptide was generated.
† Phosphorylation at Thr8 or Ser12 is not distinguishable in the present study.
‡ Phosphorylation at Tyr14 or Tyr20 is not distinguishable in the present study.