Figure 5. Time course of inactivation of PEGylated and non-PEGylated enzymes at 37 °C.

Time course of inactivation at 37 °C for EcaL-ASNase (□), R206H mutant enzyme (▲), PEG–EcaL-ASNase (■) and PEG–R206H mutant enzyme (●). The rate of inactivation was followed by periodically removing samples for assay of enzymatic activity. Results are means±S.D. for three independent experiments.