Figure 2. Immunodetection of SULTs in various parts of the gastrointestinal tract and liver using antisera raised against human SULT1C1 (upper panel) and SULT1A1 (lower panel).

Cytosolic fractions of Salmonella Typhimurium TA1538-SULT1A1 (3 μg of protein), 1A2 (5 μg), 1A3 (3 μg), 1B1 (1 μg), 1C1 (0.5 μg), 1C2 (5 μg) and human tissues (amount of protein indicated in the Figure) were electrophoresed and transferred to a nitrocellulose membrane. The blot was probed with anti-1C1-serum. Then antibodies were removed and the blot was reprobed with anti-1A1-serum. Both antisera had been pre-incubated with SULT1B1–Sepharose to abolish cross-reactivity with SULT1B1.