Figure 5. Immunodetection of SULT1B1 (left-hand panels) and SULT2A1 (right-hand panels) in colonic mucosa (upper row) and ileal mucosa (middle and lower rows).

Formalin-fixed, paraffin-embedded slices of colon and ileum were incubated with anti-1B1-serum (pre-incubated with SULT1A1–Sepharose) and anti-2A1-serum respectively, at 4 °C overnight. Bound antibodies are indicated by brown staining using the Vectastain ABC kit with diaminobenzidine as substrate. Nuclei were stained with Methyl Green.